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x1 control mosquitoes  (CH Instruments)

 
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    CH Instruments x1 control mosquitoes
    ( A, B ) P . falciparum (NF54) oocyst infection intensities of FREP1 knockout mutants at 8 days post-infection (dpi) when fed on blood with a high (0.1%) ( A ) or low (0.01%) ( B ) gametocytemia. P . falciparum oocyst infection intensity at 8 dpi in the wt <t>(X1),</t> Vasa-Cas9 (Cas9), and FREP1-gRNA lines, and FREP1 knockout mutants (FREP1-KOs). The wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) The prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes at low infection level. At low infection level, P . falciparum sporozoite loads in the salivary glands (SG) at 14 dpi ( D ) and the prevalence of the sporozoites ( E ) was significantly lower in the FREP1 knockout mosquitoes. Assays were performed with at least three biological replicates, except for ( A ) (two replicates), and equal numbers of parasites from different replicates were pooled for the dot-plots. Each dot represents the number of parasites in an individual midgut or salivary gland, and the horizontal lines (red) indicate the median values. Mann-Whitney <t>and</t> <t>Kruskal-Wallis</t> (KW) tests were used to calculate p -values and determine the significance of parasite numbers. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .
    X1 Control Mosquitoes, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x1 control mosquitoes/product/CH Instruments
    Average 90 stars, based on 1 article reviews
    x1 control mosquitoes - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection"

    Article Title: CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006898

    ( A, B ) P . falciparum (NF54) oocyst infection intensities of FREP1 knockout mutants at 8 days post-infection (dpi) when fed on blood with a high (0.1%) ( A ) or low (0.01%) ( B ) gametocytemia. P . falciparum oocyst infection intensity at 8 dpi in the wt (X1), Vasa-Cas9 (Cas9), and FREP1-gRNA lines, and FREP1 knockout mutants (FREP1-KOs). The wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) The prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes at low infection level. At low infection level, P . falciparum sporozoite loads in the salivary glands (SG) at 14 dpi ( D ) and the prevalence of the sporozoites ( E ) was significantly lower in the FREP1 knockout mosquitoes. Assays were performed with at least three biological replicates, except for ( A ) (two replicates), and equal numbers of parasites from different replicates were pooled for the dot-plots. Each dot represents the number of parasites in an individual midgut or salivary gland, and the horizontal lines (red) indicate the median values. Mann-Whitney and Kruskal-Wallis (KW) tests were used to calculate p -values and determine the significance of parasite numbers. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .
    Figure Legend Snippet: ( A, B ) P . falciparum (NF54) oocyst infection intensities of FREP1 knockout mutants at 8 days post-infection (dpi) when fed on blood with a high (0.1%) ( A ) or low (0.01%) ( B ) gametocytemia. P . falciparum oocyst infection intensity at 8 dpi in the wt (X1), Vasa-Cas9 (Cas9), and FREP1-gRNA lines, and FREP1 knockout mutants (FREP1-KOs). The wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) The prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes at low infection level. At low infection level, P . falciparum sporozoite loads in the salivary glands (SG) at 14 dpi ( D ) and the prevalence of the sporozoites ( E ) was significantly lower in the FREP1 knockout mosquitoes. Assays were performed with at least three biological replicates, except for ( A ) (two replicates), and equal numbers of parasites from different replicates were pooled for the dot-plots. Each dot represents the number of parasites in an individual midgut or salivary gland, and the horizontal lines (red) indicate the median values. Mann-Whitney and Kruskal-Wallis (KW) tests were used to calculate p -values and determine the significance of parasite numbers. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .

    Techniques Used: Infection, Knock-Out, MANN-WHITNEY

    (A, B) P . berghei (wt, ANKA 2.34) oocyst infection intensities of FREP1 knockout mutants (FREP1-KOs) at 13 days post-infection when fed on P . berghei -infected mice at high ( A ) or low ( B ) infection level. wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) At low infection level the prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes. At least three biological replicates were included in each assay, and equal numbers of mosquitoes from the different replicates were pooled for the dot-plot analysis. Each dot represents the number of parasites in an individual mosquito, and the horizontal lines (red) indicate the medians. p- values were calculated using Mann-Whitney and Kruskal-Wallis (KW) tests. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .
    Figure Legend Snippet: (A, B) P . berghei (wt, ANKA 2.34) oocyst infection intensities of FREP1 knockout mutants (FREP1-KOs) at 13 days post-infection when fed on P . berghei -infected mice at high ( A ) or low ( B ) infection level. wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) At low infection level the prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes. At least three biological replicates were included in each assay, and equal numbers of mosquitoes from the different replicates were pooled for the dot-plot analysis. Each dot represents the number of parasites in an individual mosquito, and the horizontal lines (red) indicate the medians. p- values were calculated using Mann-Whitney and Kruskal-Wallis (KW) tests. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .

    Techniques Used: Infection, Knock-Out, MANN-WHITNEY



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    x1 control mosquitoes  (CH Instruments)
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    CH Instruments x1 control mosquitoes
    ( A, B ) P . falciparum (NF54) oocyst infection intensities of FREP1 knockout mutants at 8 days post-infection (dpi) when fed on blood with a high (0.1%) ( A ) or low (0.01%) ( B ) gametocytemia. P . falciparum oocyst infection intensity at 8 dpi in the wt <t>(X1),</t> Vasa-Cas9 (Cas9), and FREP1-gRNA lines, and FREP1 knockout mutants (FREP1-KOs). The wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) The prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes at low infection level. At low infection level, P . falciparum sporozoite loads in the salivary glands (SG) at 14 dpi ( D ) and the prevalence of the sporozoites ( E ) was significantly lower in the FREP1 knockout mosquitoes. Assays were performed with at least three biological replicates, except for ( A ) (two replicates), and equal numbers of parasites from different replicates were pooled for the dot-plots. Each dot represents the number of parasites in an individual midgut or salivary gland, and the horizontal lines (red) indicate the median values. Mann-Whitney <t>and</t> <t>Kruskal-Wallis</t> (KW) tests were used to calculate p -values and determine the significance of parasite numbers. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .
    X1 Control Mosquitoes, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x1 control mosquitoes/product/CH Instruments
    Average 90 stars, based on 1 article reviews
    x1 control mosquitoes - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    ( A, B ) P . falciparum (NF54) oocyst infection intensities of FREP1 knockout mutants at 8 days post-infection (dpi) when fed on blood with a high (0.1%) ( A ) or low (0.01%) ( B ) gametocytemia. P . falciparum oocyst infection intensity at 8 dpi in the wt (X1), Vasa-Cas9 (Cas9), and FREP1-gRNA lines, and FREP1 knockout mutants (FREP1-KOs). The wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) The prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes at low infection level. At low infection level, P . falciparum sporozoite loads in the salivary glands (SG) at 14 dpi ( D ) and the prevalence of the sporozoites ( E ) was significantly lower in the FREP1 knockout mosquitoes. Assays were performed with at least three biological replicates, except for ( A ) (two replicates), and equal numbers of parasites from different replicates were pooled for the dot-plots. Each dot represents the number of parasites in an individual midgut or salivary gland, and the horizontal lines (red) indicate the median values. Mann-Whitney and Kruskal-Wallis (KW) tests were used to calculate p -values and determine the significance of parasite numbers. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .

    Journal: PLoS Pathogens

    Article Title: CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection

    doi: 10.1371/journal.ppat.1006898

    Figure Lengend Snippet: ( A, B ) P . falciparum (NF54) oocyst infection intensities of FREP1 knockout mutants at 8 days post-infection (dpi) when fed on blood with a high (0.1%) ( A ) or low (0.01%) ( B ) gametocytemia. P . falciparum oocyst infection intensity at 8 dpi in the wt (X1), Vasa-Cas9 (Cas9), and FREP1-gRNA lines, and FREP1 knockout mutants (FREP1-KOs). The wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) The prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes at low infection level. At low infection level, P . falciparum sporozoite loads in the salivary glands (SG) at 14 dpi ( D ) and the prevalence of the sporozoites ( E ) was significantly lower in the FREP1 knockout mosquitoes. Assays were performed with at least three biological replicates, except for ( A ) (two replicates), and equal numbers of parasites from different replicates were pooled for the dot-plots. Each dot represents the number of parasites in an individual midgut or salivary gland, and the horizontal lines (red) indicate the median values. Mann-Whitney and Kruskal-Wallis (KW) tests were used to calculate p -values and determine the significance of parasite numbers. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .

    Article Snippet: The prevalence of infected mosquitoes (those with at least one oocyst on the midgut) decreased by 22.3% (chi-squared test, p<0.0001, ), and the median infection intensity was reduced by 81.3% when compared to wt X1 control mosquitoes (Kruskal-Wallis test, p<0.0001, , ).

    Techniques: Infection, Knock-Out, MANN-WHITNEY

    (A, B) P . berghei (wt, ANKA 2.34) oocyst infection intensities of FREP1 knockout mutants (FREP1-KOs) at 13 days post-infection when fed on P . berghei -infected mice at high ( A ) or low ( B ) infection level. wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) At low infection level the prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes. At least three biological replicates were included in each assay, and equal numbers of mosquitoes from the different replicates were pooled for the dot-plot analysis. Each dot represents the number of parasites in an individual mosquito, and the horizontal lines (red) indicate the medians. p- values were calculated using Mann-Whitney and Kruskal-Wallis (KW) tests. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .

    Journal: PLoS Pathogens

    Article Title: CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection

    doi: 10.1371/journal.ppat.1006898

    Figure Lengend Snippet: (A, B) P . berghei (wt, ANKA 2.34) oocyst infection intensities of FREP1 knockout mutants (FREP1-KOs) at 13 days post-infection when fed on P . berghei -infected mice at high ( A ) or low ( B ) infection level. wt (X1), Cas9, and FREP1-gRNA lines served as controls. ( C ) At low infection level the prevalence of the oocyst infection was significantly lower in the FREP1 knockout mosquitoes. At least three biological replicates were included in each assay, and equal numbers of mosquitoes from the different replicates were pooled for the dot-plot analysis. Each dot represents the number of parasites in an individual mosquito, and the horizontal lines (red) indicate the medians. p- values were calculated using Mann-Whitney and Kruskal-Wallis (KW) tests. A chi-squared test was used to compare infection prevalence values. Detailed statistical analysis is presented in .

    Article Snippet: The prevalence of infected mosquitoes (those with at least one oocyst on the midgut) decreased by 22.3% (chi-squared test, p<0.0001, ), and the median infection intensity was reduced by 81.3% when compared to wt X1 control mosquitoes (Kruskal-Wallis test, p<0.0001, , ).

    Techniques: Infection, Knock-Out, MANN-WHITNEY